Effect of human β-Globin second intron on transient gene expression in mammalian cell lines
نویسندگان
چکیده
Exogenous protein expression in mammalian cells is necessary to produce therapeutic proteins and modern medical applications like developing DNA vaccines gene therapy. This study examines the human-Globin (hBG) second intron's capacity for intron-mediated enhancement (IME) various cell lines. Our study's main aim investigate effect of incorporation arrangement intron human Beta-globin into pVAX-1 cassette on improving foreign genes. Two plasmids were constructed, one with hBG positioned upstream other downstream cassette. EGFP was evaluated at mRNA levels after transfection using Lipofectamine 2000 One-way ANOVA analysis. Results showed that harbouring did not lead enhanced transient exhibit Intron Mediated Enhancement tested Further investigations are understand factors contributing lack explore alternative options optimizing
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ژورنال
عنوان ژورنال: Journal of Experimental Biology and Agricultural Sciences
سال: 2023
ISSN: ['2320-8694']
DOI: https://doi.org/10.18006/2023.11(4).663.670